Page 07/24/2014 14:50:40

New paper on assessing Symbiodinium diversity in scleractinian corals via next-generation sequencing

In this publication replicated data generated from isoclonal Symbiodinium cultures showed that all genomes contained numerous, yet mostly rare, ITS2 sequence variants. We developed an Operational Taxonomic Unit (OTU)-based pipeline for Symbiodinium ITS2 diversity typing to provisionally resolve ecologically discrete entities from intragenomic variation.
We conclude that by analyzing Symbiodinium populations in an OTU-based framework, we can improve objectivity, comparability, and simplicity when assessing ITS2 diversity in field-based studies